Research Paper Volume 15, Issue 19 pp 10105—10116

Figure 4. The expression of MMP2 is regulated by SNHG25 in CRC. (A) Volcano plot generate from the RNA-seq data showed the statistical significance (P value) and magnitude of change (fold change) of gene expression after SNHG25 knockdown. (B, C) The expression of MMP2 was detected by qRT-PCR or western blot in HCT-116 and HT-29 cells after SNHG25 knockdown. (D) GSEA results demonstrate the correlation between SNHG25 and gene sets related to metastasis (ALONSO_METASTASIS_EMT_UP, BIDUS_METASTASIS_UP and CHANDRAN_METASTASIS_TOP50_UP). (E) Representative FISH images demonstrated subcellar location of SNHG25 in HCT-116 and HT-29 cells (red). Nuclei were stained by DAPI (blue). (F) Relative SNHG25 expression in the nuclear and cytoplasm fractions of HCT-116 and HT-29 cells. Nuclear controls: U6, cytosolic controls: GAPDH. **P < 0.01 and ***P < 0.001.