Research Paper Volume 15, Issue 19 pp 10105—10116

Figure 2. PAX5 activates SNHG25 transcription in CRC. (A) The expression of SNHG25 was detected by qRT-PCR in HCT-116 and HT-29 cells transfected with PAX5 siRNAs. (B) The expression of SNHG25 was detected by qRT-PCR in HCT-116 and HT-29 cells transfected with pcDNA3.1-PAX5 or pcDNA3.1. (C) ChIP-qPCR assays were performed to detect PAX5 occupancy at the SNHG25 promoter region. Genomic DNA input was 1%. (D) Luciferase reporter assays were used to determine the PAX5 binding sites on the SNHG25 promoter region. Schematic representation of constructs for reporter is in the right panel. (E) Relative expression of SNHG25 in 90 pairs of CRC tissues and corresponding normal tissues. (F) Representative images of PAX5 immunostaining of CRC tissues and corresponding normal tissues. (G) Correlation analysis between PAX5 and SNHG25 in 90 CRC tissues (R = 0.477, p < 0.001). *P < 0.05, **P < 0.01 and ***P < 0.001.