Research Paper Volume 15, Issue 19 pp 10057—10071

Figure 6. Regulation of biological functions of ccRCC cells by miR-143-3p-ZEB1 axis. Caki cells were transfected together with ZEB1 overexpression plasmids and/or miR-143-3p mimics, and A498 cells were transfected along with ZEB1-in and/or miR-143-3p-in. (A, B) miR-143-3p and ZEB1 levels in Caki and A498 cells verified by employing qRT-PCR. (C, D) Cell proliferation was examined using CCK-8. (E, F) Migration and invasion in the cells checked by Transwell. Scale bar=100 μm. (G, H) The migration of the cells was evaluated by the wound healing test. (I, J) Cell apoptosis monitored using TUNEL. Scale bar=50 μm. (K, L) The levels of apoptosis-associated proteins were compared using WB. nsP>0.05, **P<0.01, ***P<0.001 (vs. NC or NC-in). nsP>0.05, ^^P<0.01, ^^^P<0.001 (vs. miR-143-3p /miR-143-3p-in). N=3.