Research Paper Volume 15, Issue 19 pp 10057—10071
LncRNA LINC00667 gets involved in clear cell renal cell carcinoma development and chemoresistance by regulating the miR-143-3p/ZEB1 axis
Figure 4. Regulation of ccRCC tumor growth by LINC00667. Caki cells or A498 cells with selective regulation of LINC00667 were transfused subcutaneously into the mice to construct in-vivo tumor models. (A) Plots of tumor tissues were stripped from mice on day 28 after cell injection. (B) Changes in the tumor volume. (C) The tumor mass. (D, E) LINC00667 and miR-143-3p levels in tumor tissues were ascertained by qRT-PCR. (F) The ZEB1 mRNA level in tumor tissues was gauged via qRT-PCR. (G) The ZEB1 protein level in tumor tissues was gauged via WB. (H) immunofluorescence was conducted for detecting ZEB1 in the tumor tissues. Scale bar=50 μm. (I) Ki67 profile in tumor tissues verified by IHC. The Ki67-positive cell rate was counted. nsP>0.05, **P<0.01, ***P<0.001 (vs. NC or NC-in). N=5.