Research Paper Volume 15, Issue 17 pp 9167—9181
Human adipose-derived stem cells preconditioned with a novel herbal formulation Jing Shi attenuate doxorubicin-induced cardiac damage
Figure 3. Jing Shi-preconditioned human adipose-derived stem cells (hADSCs) co-cultured with doxorubicin-challenged H9c2 cells decreased doxorubicin-induced apoptosis. (A, B) Flow cytometry analyzing cell apoptosis in H9c2 cells after doxorubicin induction with different JS-preconditioned hADSCs treatment groups versus control. Jing Shi-preconditioned human adipose-derived stem cells (hADSCs) remarkably decreased cell apoptosis in doxorubicin-challenged H9c2 cells (C, D) TUNEL assay indicating apoptotic cells (green color fluorescence) in control and different treatment groups. DAPI counter stain indicates the nucleus. The number of TUNEL positive cells decreased when doxorubicin-challenged H9c2 cells were co-cultured with Jing Shi-preconditioned hADSCs. Experiments were performed in triplicate. Data are presented as means ± SEM. *p <0.05, **p <0.01, and ***p <0.001 were significant.