Research Paper Volume 15, Issue 22 pp 12780—12793
Hypoxic BMSC-derived exosomal miR-652-3p promotes proliferation and metastasis of hepatocarcinoma cancer cells via targeting TNRC6A
Figure 2. MiR-652-3p is upregulated in hyoxic BMSCs-derived exosome and can be transferred to HCC cells. (A) Transmission electron microscopy (TMB) showed the representative image of BMSCs or hypo-BMSCs derived exosome. (B) The particle diameter of the purified exosomes was showed in histogram. (C) Exosomal markers were detected by West blotting in BMSCs derived exosome and BMSCs cells. (D) Expression of miR-652-3p was detected in exosome derived from BMSCs under different conditions using qRT-PCR. Data were presented as the mean ± SD, and analyzed with Student’s t-test; ** P < 0.01, compared with the indicated controls. (E) Expression of miR-652-3p was detected in HepG2 and SMMC-7721 cells after co-culturing with exosome derived from BMSCs under different conditions. Data were presented as the mean ± SD, and analyzed with Student’s t-test. *P < 0.05; ** P < 0.01, compared with the indicated controls.