Research Paper Volume 15, Issue 17 pp 8692—8711

Figure 5. CAP triggers GPX4-dependnet ferroptosis and creates synergies with IFNγ. (A) IFNGR2 mRNA expression in response to CAP treatment under different exposure duration. (B) TCF4 is a potential transcription factor of GPX4 as predicted using the transcription factor binding prediction tool JASPAR [36]. (C) GPX4 protein level in response to IFNγ or IFNα. (D) Relative GSH level in response to CAP, IFNγ, IFNα, or ‘CAP plus IFNγ’. (E) GPX4 protein level in response to CAP, IFNγ, or ‘CAP plus IFNγ’. (F) Relative lipid peroxidation level in response to CAP, IFNγ, or ‘CAP plus IFNγ’. Data from panel A were performed using SUM159PT cells, and panels C to F were performed using the HCT8 cell line.