Research Paper Volume 15, Issue 16 pp 8444—8457
m6A methyltransferase METTL16 mediates immune evasion of colorectal cancer cells via epigenetically regulating PD-L1 expression
Figure 5. METTL16-mediated m6A modification regulates PD-L1 expression in CRC cells. (A, B) SW480 and SW620 cells were transfected with METTL16 overexpression vectors or siRNAs, then RNA level of PD-L1 was measured by qPCR assay. (C) The interaction between METTL16 with PD-L1 mRNA was measured by RIP assay. (D, E) SW480 and SW620 cells were transfected with METTL16 overexpression vectors or siRNAs, and MeRIP assay was conducted to assess m6A enrichment. (F, G) SW480 and SW620 cells were transfected with METTL16 overexpression vectors or siRNAs and treated with RNA synthesis inhibitor α-amanitin (50 μM). The stability of PD-L1 mRNA was checked by qPCR. (H) SW480 cells were treated with CHX for indicated time points and the expression of PD-L1 was measured by western blot. **p<0.01.