Research Paper Volume 15, Issue 14 pp 7308—7323
Subcellular localization of nucleolar protein 14 and its proliferative function mediated by miR-17-5p and E2F4 in pancreatic cancer
Figure 1. Subcellular localization of NOP14 in pancreatic cancer cells. (A) Immunohistochemical localization of NOP14 in pancreatic cancer tissues. (B) Western blot analysis of NOP14 in the nucleus and cytoplasm of different pancreatic cancer cell lines. Lamin B1 was used as the nuclear reference, and GAPDH was used as the cytoplasmic reference. Standardized grey ratio was shown on the top representing relative expression of NOP14 in nucleus. (C) Immunofluorescence analysis of NOP14 in pancreatic cancer cells under magnification 200×. Blue, DAPI-labeled nuclei; green, FITC-labeled anti-NOP14 antibody; red, phalloidin-labeled cytoskeletal F-actin; merge, superimposition of the above three images. (D) Immunofluorescence analysis of RFP-NOP14 expression in transfected PANC1 cancer cells. Blue, DAPI-labeled nuclei; red, RFP or RFP-NOP14; green, nucleolar protein B23; merge, superimposition of the above three images.