Research Paper Volume 15, Issue 14 pp 6658—6689

Figure 6. Podocyte inflammaging is reduced by inhibiting NLRP3. (A–F) GSEA plots comparing the transcriptomic data from saline- and MCC950-treaded middle-aged mice for 5 hallmark gene sets (allograft rejection, IL-6/JAK/STAT3 signaling, interferon alpha response, IL-2/STAT5 signaling and complement) as well as one KEGG gene set (Nod-like receptor signaling pathway). (G–R) Immunoperoxidase staining comparing glomeruli from young mice, to middle-aged either treated with saline or MCC950 as well as middle-aged NLRP3 null mice using phospho-STAT3 (G–J), Phospho-STAT5 (K–N) or Phospho-IKKa/β (O–R). Representative images are shown; scale bars in the images correspond to 25 μm. Note that in line with the GSEA data phospho-STAT3 staining was strongly reduced by interfering with NLRP3 signaling in middle-aged mice, while STAT5 showed a more modest change.