Research Paper Volume 15, Issue 13 pp 6331—6345
MIR34A modulates lens epithelial cell apoptosis and cataract development via the HK1/caspase 3 signaling pathway
Figure 5. MIR34A modulates LECs apoptosis and cataract development through the HK1/caspase 3 signaling pathway. (A) SRA01/04 cells were transfected with MIR34A mimic and the expression of active caspase 3 at 120 h was detected by immunofluorescence. Scale bars: 100 μm. (B) SRA01/04 cells were transfected with siHK1 and the expression of active caspase 3 at 120 h was detected by immunofluorescence. Scale bars: 100 μm. (C) Lens explants of mice were co-cultured with Mir34a, siHk1, or deoxyglucose, and the expression of active caspase 3 of lens epithelium was detected by immunofluorescence. Scale bars: 20 μm.