Research Paper Volume 15, Issue 13 pp 6331—6345

Figure 3. Downregulation in HK1 expression suppresses the proliferation and induces the apoptosis of SRA01/04 cells. (A) SRA01/04 cells were transfected with siRNA HK1, and the effect of siHK1 on HK1 expression in mRNA level was detected by RT-qPCR assay. (B) SRA01/04 cells were performed as A, and the effect of siHK1 on HK1 expression at the protein level was analyzed by western blot. (C) SRA01/04 cells were performed as A, the viability of cells was assessed at 48 h, 96 h, and 120 h by CCK-8 after transfected. (D) SRA01/04 cells were performed as A, cell apoptosis was assessed at 96 h, 120 h, and 144 h and was detected by flow cytometry. (E) SRA01/04 cells were performed as A, cell apoptosis at 120 h by TUNEL assay. Scale bars: 100 μm. ^*P < 0.05; ^***P < 0.001.