Research Paper Volume 15, Issue 13 pp 6331—6345

Figure 1. MIR34A is overexpressed in cataracts, inhibiting proliferation and inducing apoptosis of SRA01/04 cells. (A) RT-qPCR analysis was performed to detect the expression of MIR34A after MIR34A mimics were transfected into SRA01/04 cells. (B) SRA01/04 cells were performed as A, the viability of cells was assessed at 48 h, 96 h, and 120 h by CCK-8 after transfected. (C) SRA01/04 cells were performed as A, and the cell apoptosis was assessed at 96 h, 120 h, and 144 h by flow cytometry. (D) SRA01/04 cells were performed as A, the TUNEL assay was performed to detect the apoptosis at 120 h. Scale bars: 100 μm. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.