Research Paper Volume 15, Issue 13 pp 6302—6330
Clinical significance, tumor immune landscape and immunotherapy responses of ADAR in pan-cancer and its association with proliferation and metastasis of bladder cancer
Figure 9. ADAR promoted bladder cancer cell proliferation, invasion and migration in vitro. (A, B) Cell proliferation assessed by CCK8 assays. Knockdown of ADAR inhibited cell proliferation in T24 and BIU87 cells. Overexpression of ADAR promoted cell proliferation in T24 and BIU87 cells. Data represent the mean ± SD from three independent experiments, *P < 0.05. (C, D) Colony formation assay showed that knockdown of ADAR significantly decreased the cloning number of T24 and BIU87 cells compared with control group, while ADAR overexpression significantly increased the cloning number of T24 and BIU87 cells. Data represent the mean ± SD from three independent experiments, *P < 0.05. (E, F) Invasion assay (Transwell) in T24 and BIU87 cell lines were measured. The results were expressed of crossing cells number per field compared with respective control. Magnification: 100×. Data represent the mean ± SD from three independent experiments, *P < 0.05. (G, H) Migration assay (Wound healing) in T24 and BIU87 cell lines were measured. Knockdown of ADAR inhibited cell migration in T24 and BIU87 cells after 24 hours. Overexpression of ADAR promoted cell migration in T24 and BIU87 cells after 24 hours. Data represent the mean ± SD from three independent experiments, *P < 0.05.