Research Paper Volume 15, Issue 12 pp 5854—5872

Figure 1. Identification of mouse iPSCs and their exosomes. (A) The cells were stained positive by immunofluorescence technique using specific antibodies for Oct4, SOX2 and SSEA-1. Oct4, octamer-binding transcription factor 4; SOX2, SRY-box transcription factor 2; SSEA-1, stage-specific embryonic antigen-1. (B) Transmission electron microscopy of iPSC-derived exosomes. (C) The concentrations and particle size of the exosomes were determined using nanoparticle tracking analysis. (D) Western blotting analysis was performed to detect the expression TSG101, HSP90, and CD81 in iPSC-derived exosomes. (E) Microscopic analysis of iPSC-derived exosomes taken up by HUVECs. Red, iPSC-derived exosomes labeled with PKH26; blue, the nuclei of HUVECs were counter-stained with DAPI. iPSCs, mouse induced pluripotent stem cells; exo, exosomes. TSG101, tumor susceptibility gene 101 protein; heat shock protein 90; CD31, cluster of differentiation molecule 31.