Research Paper Volume 15, Issue 12 pp 5279—5289
A novel peptide ‘T14’ reflects age and photo-aging in human skin
Figure 1. Specificity of T14 antibody. (A) Comparison of T14 antibody specificity using ELISA to detect peptides of various lengths with the C-terminus capped with W or K. (B) List of peptides used with amino acids sequences (epitope of the antibody -VHWK highlighted in red). (C) Dose-response of different peptides at the nanomolar range to determine the importance of the length and the epitope. (D) Dose response of T14, T30, T15 and AChE at nanomolar range. (E) Effect of trypsin on full AChE, cleaving T14 now detectable by the antibody.