Research Paper Volume 15, Issue 12 pp 5266—5278

Figure 2. Hepatic protein levels of H₂S producing enzymes. Western blotting of hepatic protein lysates quantified by densitometry analysis and expressed relative to total protein loading for (A) CSE, (B) CBS, and (C) MPST (D) TST (E) ETHE1. (F) Activity of TST protein in hepatic protein lysates as measured by a thiocyanate production capacity assay. 20 ug of liver protein lysates was loaded per well in triplicate WT data shown in black, G609G RC in pink, G609G HFD shown in green. Statistical significance was determined by Kruskal-Wallis non-parametric ANOVA test with alpha = 0.05, with Dunn's correction for multiple comparison. Grubbs outlier test with alpha = 0.05 was performed, no outliers removed. Bars show mean values with error bars representing standard error of the mean (SEM). ^*p < 0.05. Abbreviations: CSE: Cystathionine-beta-lyase; CBS: Cystathionine-Beta-synthase; MPST: 3-Mercaptopyruvate Sulfurtransferase; ETHE1: Ethylmalonic encephalopathy 1 protein; TST: Thiosulfate Sulfurtransferase.