Research Paper Volume 15, Issue 13 pp 6225—6254
An integrated bioinformatic investigation of focal adhesion-related genes in glioma followed by preliminary validation of COL1A2 in tumorigenesis
Figure 9. COL1A2 inhibits the effect of activated Jurkat cells on the viability of GBM cells in vitro. (A) Immunofluorescence staining of the nucleus, COL1A2, and CD8 in eight cases of GBM tissues. (B, C) Jurkat cells were stimulated by PMA and ionomycin, or by anti-CD3 and anti-CD28 antibodies. After 24 hours of stimulation, the expression of CD8 and CD69 proteins were detected. (D, E) The viability of U87 (D) and U251 (E) cells were analyzed by measuring luciferase activity. ***P<0.001.