Research Paper Volume 15, Issue 13 pp 6212—6224
Treatment of porcine ovarian follicles with tert-butyl hydroperoxide as an ovarian senescence model in vitro
Figure 3. The relative expression levels of Foxo1, P53, Caspase 3, and SOD after t-BHP treatment for 6 h. (A) Relative expression of mRNA level of Foxo1 in t-BHP- induced follicles. (P < 0.05). (B) Relative expression of mRNA level of P53 in t-BHP- induced follicles. (P < 0.05). (C) Relative expression of mRNA level of Caspase 3 in t-BHP- induced follicles. (P < 0.05). (D) Relative expression of mRNA level of SOD in t-BHP-induced follicles. (P < 0.05). (E) Relative protein expression levels of Foxo1, P53, Caspase 3, SOD2, and GAPDH in follicles. (F) Statistical analysis of Foxo1, P53, Caspase 3, and SOD2 in follicles. (P < 0.05).