Research Paper Volume 15, Issue 13 pp 6212—6224
Treatment of porcine ovarian follicles with tert-butyl hydroperoxide as an ovarian senescence model in vitro
Figure 2. The optimal time of follicular senescence induced by t-BHP. (A) Treating with 200 μM t-BHP, and culturing antral follicles in vitro for 1 h, 2 h, 6 h, and 12 h. Bar, 300 μm. (B) SA-β-Gal staining of granulosa cells at different time points with 200 μM t-BHP treatment. Bar, 100 μm. (C) Statistics of positive cells after different time of t-BHP treatment. P < 0.05 (D) ROS fluorescence intensity staining in follicles of each group treated with 200 μM t-BHP at different times. Bar, 5 μm. (E) Relative ROS levels after different t-BHP treatment times P < 0.05.