Figure 1.The culturing system of porcine antral follicles is feasible within 24 h. (A) The antral follicles with a diameter of 3–5 mm was selected. (B) The antral follicles were cultured in vitro for 24 and 36 h. Bar, 300 μm. (C) SA-β-Gal staining of the granulosa cells. Bar, 100 μM. (D) Statistics of positive cells at different time points. P < 0.05. (E) ROS fluorescence intensity staining of follicles in each group. Bar, 5 μm. (F) Relative ROS levels. P < 0.05.
