Research Paper Volume 15, Issue 12 pp 5734—5750

Figure 4. SNHG3 promotes BC growth and metastasis in vivo. (A) Cell cycle-related proteins CyclinD1, CDK4, and CDK6 and the metastasis-related proteins N-cadherin, vimentin, and MMP9 were detected by western blotting after SNHG3 knockdown. (B) SNHG3 expression was detected by qRT-PCR in SNHG3 stable knocked down MAD-MB-231 cells. (C) Representative image of tumors formed in nude mice and tumor volume growth curves of different groups. (D) Representative images for HE-staining, Ki67 immunostaining of tumor samples from the different groups. (E) Left panel, representative images of the gross lesion in lung tissues and hematoxylin and eosin (HE) staining of metastatic nodules in the lungs from the different groups. Right panel, the statistical result of metastatic nodule numbers in the lungs from the different groups. (F) Survival analysis of the nude mouse from different groups.