Research Paper Volume 15, Issue 12 pp 5698—5712
Sevoflurane upregulates neuron death process-related Ddit4 expression by NMDAR in the hippocampus
Figure 6. Hub genes associated with cell death biological process terms. (A) Four hub genes enriched in cell death biological process terms. (B) qPCR detection of the Ddit4 mRNA level in the hippocampus of mice with hippocampal stereotactic injection of muscimol or saline. (C) qPCR detection of the Ddit4 mRNA level in the hippocampus of mice with the hippocampal stereotactic injection of MK-801 or saline. (D) qPCR detection indicated the Ddit4 mRNA level in the hippocampus of mice that received sevoflurane exposure with NMDA (sevoflurane + NMDA group) or saline (sevoflurane group) injection into the hippocampus. Control indicates mice injected with saline in the hippocampus. MK-801 indicates mice injected with MK-801 in the hippocampus. (E) Representative pictures showed that DDIT4 level was elevated as shown by western blotting. The data shown are the means ± SDs, n = 3. nsP > 0.05, *P < 0.05.