Research Paper Volume 15, Issue 12 pp 5673—5697

Figure 7. In vitro experiments verification of DUSP10 in LGG. (A) Western blot analysis of DUSP10 expression in LGG tissues and corresponding para-carcinoma tissues. (B) Western blot and (C) qRT-PCR analysis of DUSP10 expression in NHA and LGG cell lines. (D) The cell viability of si-DUSP10-transfected and si-NC-transfected SW1088 cells by CCK-8 assays. (E, F) Effect of DUSP10 knockdown on colony formation was counted in SW1088 cells. (G, H) Representative images (G) and histogram analysis (H) of EdU assays after DUSP10 knockdown in SW1088 cells. (I, J) Cell cycle assays were implemented to evaluate the cell distribution of the SW1088 cell lines transfected with si-DUSP10 or si-NC lentiviruses. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.