Research Paper Volume 15, Issue 12 pp 5662—5672

Exploration of the effect of PUM1/Cripto-1 pathway on ferroptosis by regulating macrophage polarization in allogeneic blood transfused mice

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Figure 6. (A) The results of detecting total iron content, Fe2+ content, ROS, MDA and GSH content by corresponding test kits. The specific values were: 6.45 ± 0.35 nmol (Total iron), 3.40 ± 0.15 nmol (Fe2+), 2170.78 ± 183.53 RLU/mgprot (ROS), 15.09 ± 0.97 nmol/mL (MDA), 470.79 ± 38.25 μmol/L (GSH). (B) The results of Western blot analysis. The symbol * means p < 0.05 compared to Control group. The symbol # means p < 0.05 compared to si-PUM1 group.