Research Paper Volume 15, Issue 12 pp 5592—5610

Figure 8. Cell experiments to verify the function of key gene PGAM1. (A) After transfection with SI-PGAM1-1, PGAM1 expression in MDA-MB-231 and MDA-MB-468 cell lines was significantly down-regulated. (B) CCK-8 assay showed that PGAM1 knockdown significantly reduced the activity of both two cell lines. (C) Cloning formation assay of MDA-MB-231 cell line. PGAM1 knockdown significantly reduced the proliferation ability of breast cancer cells. (D) Cloning formation assay of MDA-MB-468 cell line. (E) Transwell experiment of MDA-MB-231 cell line. PGAM1 knockdown significantly reduced the invasion and migration of breast cancer cells. (F) Transwell experiment of MDA-MB-468 cell line. (G) Transwell statistical histogram of MDA-MB-231 cell line. (H) Transwell statistical histogram of MDA-MB-468 cell line. (I) Wound healing experiment of MDA-MB-231 cell line. After PGAM1 knockdown, the healing ability of breast cancer cells was significantly reduced. (J) Wound healing experiment of MDA-MB-468 cell line. (K) Statistical histogram of wound healing experiment of MDA-MB-231 cell line. (L) Statistical histogram of wound healing experiment of MDA-MB-468 cell line. (*p<0.05, **p<0.01, ***p<0.001).