Research Paper Volume 15, Issue 12 pp 5535—5549

Figure 3. LGALS1 regulates NCAPG at the mRNA and protein levels. Transduction efficiency confirmed by green fluorescent protein (GFP) assay in (A) SGC-7901 cells and (B) HGC-27 cells. Original magnification 200×. (C, D) Western blot confirmation of stable overexpression of Gal1 and NCAPG in (C) SGC-7901 and (D) HGC-27 cells when LGALS1 was overexpressed. (E, F) Quantitative real-time PCR (qRT-PCR) analysis of LGALS1 and NCAPG expression in (E) SGC-7901 and (F) HGC-27 cells when LGALS1 was overexpressed. Abbreviations: OE-LGALS1: Overexpression of LGALS1; NC: negative control (empty vector); NS: not significant. ^*P < 0.05; ^**P < 0.01.