Research Paper Volume 15, Issue 12 pp 5426—5444

Figure 4. Chaetocin induced the accumulation of ROS in ESCC cell. (A, B) ROS levels were analyzed by flow cytometry in TE-1 and KYSE150 cells. Results were shown as mean ± SD of three independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, ns nonsignificant compared with the control group. TE-1 and KYSE150 cells were pretreated with 5 mM NAC for 1 h and then cotreated with chaetocin (0.8 μM). (C) Apoptosis was measured by flow cytometry. Results were shown as mean ± SD of three independent experiments. ^**P < 0.01, ^***P < 0.001, ^****P < 0.000. (D) Expression levels of PARP, Cleaved PARP, caspase-3, cleaved-caspase-3, Bax and Bcl-2 were detected by western blot. GAPDH was used as the loading control.