Research Paper Volume 15, Issue 12 pp 5426—5444

Figure 2. Chaetocin triggers mitotic arrest in ESCC cells. (A) TE-1 and KYSE150 cells were treated with the indicated concentrations of chaetocin (0, 0.2, 0.4 and 0.8 μM) for 24 h. The cell cycle distribution was analyzed using flow cytometry. The values indicate the mean ± SD of three independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 compared with the control (one-way analysis of variance). (B) Protein expression of p-CDK1 and p-histone h3 was detected using western blot analysis. GAPDH was used as the loading control. Blots presented here are representative of three independent experiments. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 compared with the control group.