Research Paper Volume 15, Issue 12 pp 5412—5425
Tumor-suppressive function and mechanism of miR-873-5p in glioblastoma: evidence based on bioinformatics analysis and experimental validation
Figure 5. miR-873-5p inhibits GBM cell proliferation and invasion by inhibiting the HMOX1/HIF1α/SPOP signaling axis. T98G cells were transfected with miR-873-5p mimic alone or combined with oe-SPOP. (A) Expression of miR-873-5p, HIF1α and SPOP in T98G cells determined by RT-qPCR. (B) Western blot of HIF1α and SPOP proteins in T98G cells. (C) Western blot of the CCND1, E-cadherin, MYC, MMP-7, and N-cadherin proteins in T98G cells. (D) Proliferation of T98G cells measured by CCK-8 assay. (E) Invasion of T98G cells measured by Transwell assay. (F) Western blot of HMOX1, HIF1α, and SPOP proteins in T98G cells transfected with miR-873-5p mimic alone or combined with oe-HMOX1 or oe-HIF1α. *p < 0.05, compared to NC-mimic + oe-NC-transfected T98G cells. #p < 0.05, compared to miR-873-5p mimic + oe-NC-transfected T98G cells. The cell experiment was run in triplicate independently.