Research Paper Volume 15, Issue 13 pp 6100—6116

Figure 7. Effects of MCF2L-AS1-miR-33a-5p-FGF2 on MHCC97H cell proliferation, invasion and apoptosis. miR-33a-5p mimics or sh-FGF2 or MCF2L-AS1 overexpression plasmids were transfected into MHCC97H cells. (A, B) qRT-PCR detected MCF2L-AS1and miR-33a-5p expression levels. (C) qRT-PCR checked the mRNA level of FGF2. (D) Western blot was used for detecting FGF2 expression. (E) CCK8 assay detected MHCC97H and HCCLM3 cell proliferation. (F, G) EdU staining was performed for evaluating cell proliferation. Scale bar=50 μm. (H) Colony formation assay was conducted for detecting cell colony formation ability. (I) Western blot was conducted for evaluating apoptosis-related proteins, including Bcl2, Bcl-xl, and Bax. (J, K) Transwell assay detected MHCC97H cell migration and invasion, Scale bar=200 μm. NS P>0.05, * P<0.05, **P<0.01, *** P<0.001. N=3.