Research Paper Volume 15, Issue 13 pp 6100—6116
MCF2L-AS1 promotes the biological behaviors of hepatocellular carcinoma cells by regulating the miR-33a-5p/FGF2 axis
Figure 2. Effects of MCF2L-AS1 on cell proliferation, invasion and apoptosis. MCF2L-AS1 overexpression plasmids were transfected into MHCC97H and HCCLM3 cells. (A, B) MCF2L-AS1 profile was detected through qRT-PCR. (C, D) CCK8 assay detected MHCC97H and HCCLM3 cell proliferation. (E) Colony formation assay was conducted for detecting cell colony formation ability. (F) EdU staining was performed for evaluating cell proliferation. Scale bar=50 μm. (G, H) MHCC97H and HCCLM3 cell migration and invasion were tracked by Transwell. Scale bar=200 μm. * P<0.05, **P<0.01, *** P<0.001. N=3.