Figure 4. Plasmid transfection efficiency and detection of its activity. (A) Fluorescence of plasmid transfection at 24,48,72 h; (B) 48 hours sh260 CRYAA expression amount compared with the normal group, with statistical significance, * p<0.05. (C) Cell activity at three time periods 24, 48, and 72 after transfection, OD value of sh260 group compared with the normal group, with statistical significance, ** p<0.05, * p<0.05. There was no difference between ser and con group.