Figure 4.The interaction among lncRNA H19, miR-194, and E2F3. (A, B) Starbase database the binding site between lncRNA H19 and miR-194 as well as the binding site between miR-194 and E2F3. (C–F) qRT-PCR examined the profiles of lncRNA H19 and miR-194. (G, H) Dual luciferase reporter gene assay measured luciferase activity of DU145 cells. (I, J) The miR-194 level was probed via qRT-PCR. (K–R) Western blot and qRT-PCR tested the effects of lncRNA H19 and miR-194 on E2F3 expression. ns P > 0.05, ***P < 0.001, n = 3.
