Research Paper Volume 15, Issue 9 pp 3331—3355
In vitro and in vivo effects of zoledronic acid on senescence and senescence-associated secretory phenotype markers
Figure 5. Cytometry by time of flight (CyTOF) reveals the cellular targets of zoledronic acid treatment. (A) CyTOF of hematopoietic cells following 2 weeks of zoledronic acid treatment led to distinct clusters. A t-distributed Stochastic Neighbor Embedding (tSNE) visualization of vehicle treated specimens (n=10) shows the integration of the datasets with the arrow pointing to the CD115+/CD3e-/Ly6G-/CD45R- cells. (B) p16 expression and (C) p21 expression in a tSNE visualization in the Veh group demonstrates a high expression of p16 in the CD115+ cells.vs. (D) Heatmap showing the expression of senescence, SASP, and identity markers in the bone marrow hematopoietic cells, demonstrating that the CD115+/CD3e-/Ly6G-/CD45R- cells express the highest levels of p16, pATM (DNA damage marker), and SASP proteins.