Research Paper Volume 15, Issue 9 pp 3635—3643

Figure 3. TIMP2 can aggravate neuronal apoptosis in CIRI mice. (A, B) TUNEL staining is performed to observe neuronal apoptosis in the control, I/R, and I/R+TIMP2 groups. In the control group, there is basically no green fluorescence, while the number of TUNEL-positive cells is larger in the I/R and I/R+TIMP2 groups compared with that in the control group. Furthermore, the number of TUNEL-positive cells is significantly larger in the I/R+TIMP2 group than that in the I/R group. In contrast, the number of TUNEL-positive cells is significantly smaller in the TIMP2-KD group than that in the I/R group. These results suggested that TIMP2 may aggravate neuronal apoptosis in mice with CIRI. ^**P < 0.01, ^*P < 0.05.