Research Paper Volume 15, Issue 9 pp 3621—3634
Ling Zhi-8, a fungal immunomodulatory protein in Ganoderma lucidum, alleviates CPT-11-induced intestinal injury via restoring claudin-1 expression
Figure 2. Restoration of claudin-1 mRNA expression by pretreatment of LZ-8 in IEC-6 cells following CPT-11 treatment, assessed by qPCR analysis. After normalization with β-actin, relative expression levels were shown with the mean value of untreated cells in the control group set at 1. LZ-8 treatment had no obvious influence on the expression levels of claudin-1 mRNA in IEC-6 cells, but CPT-11-treated IEC-6 cells expressed lower levels in a dose-dependent manner. LZ-8 pretreatment increased claudin-1 mRNA expression in IEC-6 cells following CPT-11 treatment. Data were presented as mean ± SD, and one-way ANOVA was used for statistical analysis. * indicates P < 0.05, compared with the untreated cells; # indicates P < 0.05, compared with the CPT-11-treated cells. n = 5.