Research Paper Volume 15, Issue 9 pp 3621—3634

Figure 1. Protective effects of LZ-8 against CPT-11-induced cytotoxicity in IEC-6 cells. CCK-8 assay was used to determine the viability of IEC-6 cells. For control, the absorbance of untreated cells was considered 100%. LZ-8 treatment had no obvious influence on the viability and morphology of IEC-6 cells, but cell death in a dose-dependent manner was noted in IEC-6 cell treated with CPT-11. LZ-8 pretreatment improved the cell viability and morphology of IEC-6 cells following CPT-11 treatment. Data were presented as mean ± SD, and one-way ANOVA was used for statistical analysis. * indicates P < 0.05, compared with the untreated cells; # indicates P < 0.05, compared with the CPT-11-treated cells. n = 5. (A) The viability of IEC-6 cells after 24-hour incubation of LZ-8 (left panel), 24-hour incubation of CPT-11 (middle panel), or 24-hour pretreatment of LZ-8 following 24-hour incubation of CPT-11 (right panel). (B) The viability of IEC-6 cells after 48-hour incubation of LZ-8 (left panel), 48-hour incubation of CPT-11 (middle panel), or 24-hour pretreatment of LZ-8 following 48-hour incubation of CPT-11 (right panel). (C) Effects of LZ-8 on CPT-11-induced cell morphological changes.