Research Paper Volume 15, Issue 9 pp 3465—3479

Figure 5. Overexpression of JDP2 inhibited the miR-143-5p–induced EMT in ARPE-19 cells. (A–D) ARPE-19 cells were transfected with miR-NC alone, miR-143-5p mimics alone, pcDNA 3.1 plasmid alone, pcDNA 3.1-JDP2 plasmid alone, pcDNA 3.1-JDP2 plasmid together with miR-143-5p mimics, or pcDNA 3.1-JDP2 plasmid together with miR-143-5p mimics and followed by PA treatment. (A) Relative levels of miR-143-5p and JDP2 mRNA in ARPE-19 cells in different treatment groups were determined via quantitative PCR. (B, C) Migration of ARPE-19 cells in different treatment groups was determined via wound healing assay. (D) Western blot assays determined the protein expression of E-cadherin, Vimentin, Snail, α-SMA, and Fibronectin in ARPE-19 cells in different treatment groups (n = 3 for each group). ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001, as compared with the indicated group.