Research Paper Volume 15, Issue 9 pp 3442—3464
Identification and validation of immunogenic cell death-related score in uveal melanoma to improve prediction of prognosis and response to immunotherapy
Figure 10. Role of PARP8 in uveal melanoma cells. (A, B) The expression level of PARP8 was positively correlated with the ICDscore in patients from TCGA-UVM (A) and GSE22138 (B) cohorts. (C, D) Kaplan-Meier analysis showed that patients with high PARP8 expression had a longer OS in TCGA-UVM (C) and GSE22138 (D) cohorts. (E, F) CCK-8 assay showed that PARP8 knockdown in MUB2B or C918 cell inhibited cell proliferation. (G, H) CCK-8 assay showed that PARP8 knockout in MUB2B and C918 inhibited cell proliferation. (I, J) In TCGA-UVM and GSEGSE22138 cohorts, PARP8 was positively correlated with CD274 expression. (K, L) qPCR assay showed that PARP8 knockout in MUB2B and C918 could inhibit CD274 expression. Abbreviation: Ns: not significant. *p < 0.05; **p < 0.01; ***p < 0.001.