Research Paper Volume 15, Issue 8 pp 3035—3051

Figure 3. Construction of drug-resistant cell lines and alteration of TYRO3 expression. (A) TYRO3 expression in CRC cell lines searched from the CCLE platform. (B) TYRO3 expression in 5 CRC cell lines selected by CCLE platform. (C) TYRO3 expression in 5 CRC cell lines evaluated by Western blot. (D) Immunoblot result of TYRO3 expression in 5 CRC cell lines quantified by ImageJ. (E) The viability of H-WT and H-DR treated with different concentrations of 5-Fu for 24 hours. (F) The IC50 of H-WT and H-DR treated with 5-Fu. (G) The viability of T-WT and T-DR treated with different concentrations of 5-Fu for 24 hours. (H) The IC50 of T-WT and T-DR treated with 5-Fu. (I) Western blot detecting the change of TYRO3 protein levels in wild type and drug-resistance CRC cells. (J) Immunoblot result of wild type and drug-resistance CRC cells semi-quantified by ImageJ. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.