Research Paper Volume 15, Issue 8 pp 2999—3020

Figure 9. Rasgrf1 upregulation by the inhibition of miR-323-3p could improve the synapse density decrease after CCH. (A) The synapses were observed with uranyl acetate negative staining under transmission electron microscope; (Con (n = 3), 2VO (n = 3), 2VO+miRNA-323-3p antagomir (n = 3), 2VO+miRNA-323-3p antagomir NC (n = 3), 2VO+miRNA-323-3p antagomir + RasGRF1 siRNA (n = 3), 2VO+miRNA-323-3p antagomir + RasGRF1 scramble siRNA (n = 3)). (B) Synapses densities were calculated. (C and D) The cumulative and specific distribution of synapses density was calculated. Scale bar = 200 nm. ^**p < 0.01. All of the experiments were repeated three times.