Research Paper Volume 15, Issue 8 pp 2999—3020

Figure 8. Rasgrf1 upregulation by the inhibition of miR-323-3p could ameliorate the neural structural plasticity impairment after CCH. (A) The dendritic spines were observed with Golgi staining. (B) The cumulative distribution of dendritic spines density was calculated; (Con (n = 1), 2VO (n = 1), 2VO+miRNA-323-3p antagomir (n = 1), 2VO+ miRNA-323-3p antagomir NC (n = 1), 2VO+miRNA-323-3p antagomir + RasGRF1 siRNA (n = 1), 2VO+miRNA-323-3p antagomir + RasGRF1 scramble siRNA (n = 1)). (C) The total dendritic spines density. (D) The cumulative distribution of dendritic spines density. (E) The mushroom dendritic spines density was counted and calculated. Scale bar = 5 μm. ^**p < 0.01. All of the experiments were repeated three times.