Research Paper Volume 15, Issue 8 pp 2999—3020

Figure 6. Rasgrf1 upregulation by the inhibition of miR-323-3p could improve the spatial cognitive. dysfunction after CCH. MWM was employed to detect the spatial cognitive abilities. (A) The latency to reach the platform during the trail training was recorded and analyzed. (B) After 1 day-rest, the latency to reach the platform. (C) The crossing times around the platform, and (D) staying time in platform quadrant were recorded and analyzed. The Y maze was employed to further test the cognitive abilities. (E) The number of arms entries alteration, (F) percent of arms entries alteration, and (G) total arms entries times were recorded and analyzed. Con: sham group, n = 12; 2VO: bilateral common arteries occlusion group, n = 15; 2VO+miR-323-3p antagomir: bilateral common arteries occlusion group with miR-323-3p antagomir intra-cerebroventricular injection (ICV) injection, n = 15; 2VO+miR-323-3p NC: 2VO group with ICV injection of miR-323-3p antagomir negative control, n = 15; 2VO+miR-323-3p antagomir+RasGRF1 siRNA: 2VO group with ICV injection of miR-323-3p antagomir and RasGRF1 siRNA, n = 15; 2VO+miR-323-3p antagomir + RasGRF1 scramble: 2VO group with ICV injection of miR-323-3p antagomir and RasGRF1 siRNA scramble, n = 15. ^*p < 0.05, ^**p < 0.01. All of the experiments were repeated three times.