Research Paper Volume 15, Issue 8 pp 2999—3020

Figure 5. The inhibition of miR-323-3p could up-regulate the expression of RasGRF1 in hippocampus after CCH. (A) The RasGRF1 expression, the total and phosphorylated ERK expression were assayed by Western blot. β-actin was as inner control for samples loading normalization. (Con (n = 3), 2VO (n = 3), 2VO+miRNA-323-3p antagomir (n = 3), 2VO+ miRNA-323-3p antagomir NC (n = 3), 2VO+miRNA-323-3p antagomir + RasGRF1 siRNA (n = 3), 2VO+miRNA-323-3p antagomir + RasGRF1 scramble siRNA (n = 3)). (B) The relative expression level of RasGRF1 was calculated and analyzed statistically. (C) The p-ERK relative expression level was calculated and analyzed statistically. Relative intensity of p-ERK level is calculated by p-ERK/total ERK. ^**p < 0.01. The experiments were repeated three times.