Research Paper Volume 15, Issue 9 pp 3394—3409

Figure 8. miR-379-5p reduces the proliferation and migration ability of gastric cancer by targeting GRB10. (A) Diagram of the GRB10 mRNA and miR-379-5p binding site. Abbreviation: KD: knockdown group. (B) The relative luciferase activity was measured with a dual-luciferase reporter assay. (C) RIP assay was conducted using an antibody against Ago2. (D) The relative expression of miR-379-5p was detected by qRT-PCR. (E) The expression of GRB10 was detected by Western Blot. (F) The relative expression of GRB10 mRNA was detected by qRT-PCR. (G) MiR-379-5p expression in the TCGA database. (H) Cell proliferation was determined by the CCK-8 assay. (D) Effects of miR-379-5p on cell adhesion ability. (I) Cell migratory ability detected by Transwell migration assay. (J) Effects of miR-379-5p on cell adhesion ability. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.