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Research Paper Volume 15, Issue 10 pp 4071—4083
lncRNA PITPNA-AS1 promotes cell proliferation and metastasis in hepatocellular carcinoma by upregulating PDGFD
Figure 5. PITPNA-AS1 promotes HCC progression through the miR-363-3p/PDGFD axis. (A) Protein level of PDGFD in Huh7 and Hep3B cells transfected with pcDNA3.1-PDGFD. (B) Cell proliferation of HCC cells transfected with sh-NC, sh-PITPNA-AS1#1, sh-PITPNA-AS1#1+miR-363-5p inhibitor, or sh-PITPNA-AS1#1+miR-363-5p inhibitor + pcDNA3.1-PDGFD was examined by the CCK-8 assay. (C and D) Cell migration and invasion of Huh7 and Hep3B cells transfected with sh-NC, sh-PITPNA-AS1#1, sh-PITPNA-AS1#1+miR-363-5p inhibitor, or sh-PITPNA-AS1#1+miR-363-5p inhibitor+pcDNA3.1-PDGFD was investigated by the transwell assay. **P < 0.01, compared to the sh-NC group; ^^P < 0.01, compared to the sh-PITPNA-AS1#1 group. (E) EMT-related protein levels of Huh7 and Hep3B cells transfected with sh-NC, sh-PITPNA-AS1#1, sh-PITPNA-AS1#1+miR-363-5p inhibitor, or sh-PITPNA-AS1#1+miR-363-5p inhibitor+pcDNA3.1-PDGFD were determined by western blot.