Research Paper Volume 15, Issue 17 pp 8613—8629
NRP1 regulates autophagy and proliferation of gastric cancer through Wnt/β-catenin signaling pathway
Figure 11. Validation of NRP1’s regulation of tumor autophagy and proliferative capacity in vitro. (A) QPCR was used to detect NRP1 gene expression in gastric cancer cell lines. (B) western blot analysis. (C, D) EdU assay and cell immunofluorescence were used to detect the effect of NRP1 knockout on cell proliferation. (E) Autophagy assay to detect the effect of NRP1 knockdown on autophagy. (F) The effect of NRP1 knockdown on autophagy was detected by transmission electron microscopy. (*P< 0.05; **P< 0.01; ***P< 0.001;****P< 0.0001).