Research Paper Volume 15, Issue 3 pp 718—733
Interleukin 6 promotes BMP9-induced osteoblastic differentiation through Stat3/mTORC1 in mouse embryonic fibroblasts
Figure 6. Role of Raptor in the effect of IL-6 on promoting osteogenic differentiation inf MEFs. (A) Effect of BMP9 and/or IL-6 on Raptor protein level was measured using western blot assay. (B) Effect of BMP9 and/or IL-6 on Rictor protein level was measured using western blot assay. (C) Effect of BMP9 and/or IL-6 knockdown on Raptor protein level was measured using western blot assay. (D) Effect of BMP9 and/or IL-6 knockdown on Rictor protein level was measured using western blot assay. (E) Quantification of western blot assay shows the effect of BMP9 and/or IL-6 on Raptor protein level. (F) Quantification of western blot assay shows the effect BMP9 and/or IL-6 on Rictor protein level. (G) Quantification of western blot assay shows the effect of BMP9 and/or IL-6 knockdown on Raptor protein level. (H) Quantification of western blot assay shows the effect of BMP9 and/or IL-6 knockdown on Rictor protein level. (I) Effect of IL-6 and/or Raptor knockdown on total mTOR and p-mTOR level was measured using western blot assay. (J) Quantification of western blot assay shows the effect of IL-6 and/or Raptor knockdown on total mTOR and p-mTOR level. (K) Effect of IL-6 and/or Raptor knockdown on RUNX2 protein level was measured using western blot assay. (L) Quantification of western blot assay shows the effect of IL-6 and/or Raptor knockdown on RUNX2 protein level. (M) Effect of IL-6 and/or Raptor knockdown on ALP activity was measured using histochemical stain. (N) Quantification of histochemical stain shows the effect of IL-6 and/or Raptor knockdown on ALP activity. (** P < 0.01).