Research Paper Volume 15, Issue 3 pp 718—733
Interleukin 6 promotes BMP9-induced osteoblastic differentiation through Stat3/mTORC1 in mouse embryonic fibroblasts
Figure 4. Role of mTOR in the effect of IL-6 on promoting osteogenic differentiation in MEFs. (A) Effect of BMP9, IL-6, and/or IL-6 knockdown on mTOR phosphorylation (p-mTOR) was measured using immunofluorescent stain and confocal assay. (B) Effect of BMP9 and/or IL-6 on total mTOR and p-mTOR level was measured using western blot assay. (C) Quantification of western blot assay show the effect of BMP9 and/or IL-6 on total mTOR and p-mTOR. (D) Effect of BMP9 and/or IL-6 knockdown on total mTOR and p-mTOR level was measured using western blot assay. (E) Quantification of western blot assay show the effect of BMP9 and/or IL-6 knockdown on total mTOR and p-mTOR. (F) Effect of IL-6 and/or rapamycin on BMP9-induced RUNX2 protein level was measured using western blot assay. (G) Effect of IL-6 and/or rapamycin on BMP9-induced ALP activity was measured using histochemical stain. (Rapamycin: mTOR inhibitor). (** P < 0.01).